Category |
Enzymes |
Clonality |
N/A |
Raised in |
N/A |
Target |
Reverse Transcriptase (cDNA up to 8 kb |
Immunogen |
see included datasheet or contact us |
Species reactivity |
Human, Mouse, Rat (for other species please inquire) |
Purification |
Affinity purified |
Applications |
WB (for other applications please contact us) |
Stock availability |
Shipped within 5-10 working days. |
Storage conditions |
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
Footnote |
This product is for research use only. |
Tissue |
cdna |
Description |
this 1 cDNA is double stranded complementary DNA derived by abbex from mRNA with very high fidelity reverse transcription using a pfu or pfx mix,Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs. |
Goup |
reverse transcription |