| Estimated Turnaround Time |
6-11 business days |
| Target Name |
NA |
| Target's alterntive name |
CFB |
| Species Reactivity |
Human |
| Sample Type |
Serum, Plasma, Biological Fluids |
| Detection Range |
3.13-200 ng/mL |
| Sensitivity |
1.88 ng/mL |
| Precision |
NA |
| Detection Method |
Colorimetric |
| Assay Time |
4.5 hours |
| Application |
For research use only. Not for diagnostic procedures. |
| Assay Type |
Sandwich |
| Shipping Conditions |
Ice packs |
| Storage Temperature |
Short term: 4°C; Long term: see manual. |
| Precaution of Use |
The Stop Solution is acidic. Do not allow to contact skin or eyes. |
| Shelf life |
Use Human CFB (Complement Factor B) ELISA Kit before 6 months |
| Specificity |
NA |
| Cross Activity |
No significant cross-reactivity or interference was observed. |
| Test Principle |
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFB . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CFB. The concentration of Human CFB in samples can be calculated by comparing the OD of the samples to the standard curve. |
| Stability |
NA |
| Quality Systems |
The kit is manufactured at ISO 9001 certified facilities. |
| Research Area |
Immunology |
| Properties |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. |
| Test |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Description |
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. |